Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates
نویسندگان
چکیده
منابع مشابه
Epigenetic reprogramming by somatic cell nuclear transfer in primates.
We recently demonstrated that somatic cells from adult primates could be reprogrammed into a pluripotent state by somatic cell nuclear transfer. However, the low efficiency with donor cells from one monkey necessitated the need for large oocyte numbers. Here, we demonstrate nearly threefold higher blastocyst development and embryonic stem (ES) cell derivation rates with different nuclear donor ...
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Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
متن کاملReprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling.
BACKGROUND Somatic cell nuclear transfer (SCNT) requires cytoplast-mediated reprogramming of the donor nucleus. Cytoplast factors such as maturation promoting factor are implicated based on their involvement in nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC). Given prior difficulties in SCNT in primates using conventional protocols, we hypothesized that the ability...
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Nuclear transfer, also referred to as cloning, attracted public attention when the first mammal, the sheep Dolly, was cloned from an adult cell. In this review, we highlight some of the milestones reached in the 50 years following the first nuclear transplantations in frogs. We also address the problems associated with nuclear transfer and provide an survey on current stem-cell technology. In t...
متن کاملI-8: Somatic Cell Nuclear Reprogramming byMouse Oocytes Endures Beyond ReproductiveDecline
Background: The mammalian oocyte has the unique feature of supporting fertilization and normal development while being able of reprogramming the nuclei of somatic cells towards pluripotency, and occasionally even totipotency. Whilst oocyte quality is known to decay with somatic ageing, it is not a given that different biological functions decay concurrently. In this study, we tested whether ooc...
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ژورنال
عنوان ژورنال: Stem Cells
سال: 2009
ISSN: 1066-5099,1549-4918
DOI: 10.1002/stem.60